Composite

Part:BBa_M36704

Designed by: Katie Lund   Group: Stanford BIOE44 - S11   (2011-05-06)


PaceBAK with Gemini

PaceBAK, the IclR associated promoter region of the aceBAK operon, has been coupled with the Gemini protein generator. PaceBAK includes numerous binding sites for IclR, which, when actively bound by pyruvate-stabilized IclR, will inhibit expression of Gemini.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109


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Parameters
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